ISHS Acta Horticulturae 538: Eucarpia symposium on Fruit Breeding and Genetics
CULTIVAR IDENTIFICATION IN SWEET CHERRY (Prunus avium L.) USING AFLP AND MICROSATELLITE MARKERS |
| Authors: | M. Boritzki, J. Plieske, D. Struss |
| Keywords: | identification key, molecular marker, genetic variation |
| Abstract:
Ten AFLP primer pair combinations were used to clearly separate 128 sweet cherry accessions using the Neighbour–Joining–analysis method. One hundred and twenty-four of 712 AFLP fragments were polymorphic among the accessions. The number of polymorphic loci detected per AFLP primer pair ranged from 5 to 26. 188 sweet cherry accessions were also analysed using 13 microsatellite primers. The resulting fragments and fragment patterns were combined into profiles. The resolution of the computed Neighbour-Joining dendrogram was higher compared with the AFLP dendrogram. In order to develop an identification key for each investigated sweet cherry accession, the data were analysed separately for each microsatellite marker. The marker showing the highest variation among accessions was chosen as ’first marker’. The remaining markers were used to separate the accessions in the groups created by the first marker until all accessions were clearly identified. Using this microsatellite based key we created a powerful tool for sweet cherry cultivar identification, which could be the starting point for the development of a marker assisted cultivar identification database. | |
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