ISHS Acta Horticulturae 538: Eucarpia symposium on Fruit Breeding and Genetics
CHARACTERIZATION OF PEAR SPECIES AND CULTIVARS USING RAPD PRIMERS |
| Authors: | M.M. Sharifani, J.F. Jackson |
| Keywords: | molecular markers, Pyrus, PCR, polymorphism |
| Abstract:
Random 10-mer (RAPD) primers were employed to identify 16 pear species, cultivars, and few progenies. The progenies were obtained from cross pollination of two pear male parents with the ‘Packham Triumph’ female receptive in a program of pollen flow study. Nine of twenty random decamer primers produced useful band markers (OPA08, OPA10, OPA12, OPB12, OPC06, OPC07, OPD01, OPD02 and OPD010). The results indicate that pear cultivars and species can be identified by using few of the above primers. Progenies were screened using the above nine primers. Only primers OPC06 and OPC07 were useful to discriminate among the progenies. The other primers in this respect did not produce distinct marker bands or polymorphism patterns. In conclusion, employing several primers are needed to screen differences among progenies and parents in pear. | |
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