ISHS Acta Horticulturae 538: Eucarpia symposium on Fruit Breeding and Genetics
GENOME INVESTIGATION IN SOUR CHERRY, P. CERASUS L. |
| Authors: | M. Schuster, H. Schreibner |
| Keywords: | P. cerasus, P. avium, P. fruticosa, genomic in situ hybridization, karyotype, |
| Abstract:
Mitotic chromosome preparations of the sour cherry ‘Schattenmorelle’ were used to study the genome constitution of Prunus cerasus L. by genomic in situ hybridization (GISH) and karyotype analysis. Total genomic DNA from the presumably donor species P. avium and P. fruticosa were used as the probes. The probes were labelled with Digoxigenin-11-dUTP by nick translation. The probe DNA’s of P. avium and of P. fruticosa each hybridized to 16 of the 32 chromosomes of sour cherry. By means of the karyotype analysis of C-banded P. cerasus and P. avium chromosomes, it was possible to detect the P. avium chromosomes in the P. cerasus genome. These results confirmed that P. avium and P. fruticosa are the donor species of the P. cerasus genome. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
