ISHS Acta Horticulturae 538: Eucarpia symposium on Fruit Breeding and Genetics
EVALUATION OF A SAND-BRAN INOCULATION TECHNIQUE FOR ASSESSMENT OF RESISTANCE OF APPLE SEEDLINGS AGAINST PHYTOPHTHORA CACTORUM (LEBERT & COHN) SCHROETER |
| Authors: | P.T. Zondo, I.F. Labuschagné, C. Linde, S. Denman |
| Keywords: | Phytophthora, aggressiveness, crown rot, apples, inoculation technique |
| Abstract:
A sand-bran inoculation technique was evaluated to assess apple (Malus x domestica Borkh.) seedlings resistance to collar, crown and root rot caused by Phytophthora cactorum. Root dry weight and shoot height were used as a measure of resistance. Five P. cactorum isolates were tested. Inoculum was mixed with sterile planting medium to make up mass (inoculum):mass (planting medium) concentrations of 0.0, 0.5, 1.0, 2.0, 5.0, and 10.0% inoculum levels. Thirteen week-old cv. Jonathan seedlings were transplanted into the inoculated soil and kept in a greenhouse for 13 weeks at 25 °C with daily watering. All tested isolates were pathogenic to seedlings but differed significantly in their levels of aggressiveness. The degree of disease at varying inoculum concentrations did not differ significantly. | |
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