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ISHS Acta Horticulturae 535: First International Citrus Biotechnology Symposium

GENETIC POLYMORPHISM OF SWEET ORANGE (Citrus sinensis [L.] Osbeck) VARIETIES EVALUATED BY RANDOM AMPLIFIED POLYMORPHIC DNA

Authors:   M.L.P. Natividade Targon, M.A. Machado, H.D. Coletta Filho, M. Cristofani
Keywords:   sweet orange, RAPD, fingerprint, molecular marker
DOI:   10.17660/ActaHortic.2000.535.5
Abstract:
Genomic DNA was extracted from sweet orange (Citrus sinensis [L.] Osbeck) varieties in the germplasm collection of the Centro de Citricultura Sylvio Moreira, Instituto Agronomico, Cordeirópolis, SP, Brazil. Representatives of each sweet orange group were assayed using random amplified polymorphic DNA (RAPD) to characterize and detect polymorphisms among the varieties. In spite of phenotypic differences, no polymorphisms were detected. We concluded that RAPD analysis is not applicable in differentiation of sweet orange varieties. More sensitive methods of analysis should be used.

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