ISHS Acta Horticulturae 535: First International Citrus Biotechnology Symposium
PRELIMINARY CHARACTERIZATION OF SEVERAL DOUBLED HAPLOIDS OF CITRUS CLEMENTINA CV. NULES |
| Authors: | M.A. Germanà, F.G. Crescimanno, G. Reforgiato Recupero, M.P. Russo |
| Keywords: | haploid, anther culture, isozyme, RAPD |
| Abstract:
Haploid embryogenic calli and plantlets of Citrus clementina Hort. ex Tan., cv. Nules were obtained from anther culture in 1990 (Germanà et al., 1994). Since then, several studies have been carried out to improve the frequency with which microspores form embryos and the percentage of successful plants transferred from in vitro to in vivo. Although haploid embryos of clementine germinate vigorously, haploid plantlets grow slowly in soil, presumably because of inbreeding depression. Better results have been obtained by grafting the homozygous shoots onto Troyer citrange seedlings and, after several months, grafting the new shoots onto 2-year-old sour orange seedlings. Such homozygous plants show differences in leaf morphology and habitus. The aim of this research was to characterize several homozygous calli, plantlets, and plants from morphological and molecular points of view, using isozymes and random amplified polymorphic DNA (RAPD) markers. | |
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