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ISHS Acta Horticulturae 535: First International Citrus Biotechnology Symposium

DEVELOPMENT OF MOLECULAR MAPS FOR ROOTSTOCK BREEDING IN CITRUS

Authors:   G. Reforgiato Recupero, M.P. Russo, M. De Simone, A. Natoli, P. Ajmone Marsan, A. Marocco
Keywords:   AFLP, RAPD, RFLP, linkage maps
Abstract:
Citrus breeding is a lengthy process complicated by a long juvenile period, heterozygosity, and nucellar embryony. The advent of molecular markers now permits the construction of accurate linkage maps, the investigation and mapping of agronomic traits using quantitative trait loci (QTLs), and marker-assisted selection. Here we report data on Citrus aurantium and C. latipes molecular maps based on a two-way pseudo-testcross mapping strategy using amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), and restriction fragment length polymorphism (RFLP) markers. Further application of these maps is envisaged in the localisation of QTLs controlling nucellar embryony.

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