ISHS Acta Horticulturae 535: First International Citrus Biotechnology Symposium
CLONING AND CHARACTERIZATION OF TWO CITRUS MITOGEN-ACTIVATED PROTEIN KINASE GENES AND EFFECTS OF ETHYLENE ON THEIR EXPRESSION PATTERNS |
| Authors: | G.Y. Zhong, R. Goren, J. Riov, D. Holland |
| Keywords: | mitogen-activated protein kinase, ethylene, wounding |
| Abstract:
The influence of the simple gas ethylene on the activity of a Citrus myelin basic protein kinase was investigated. Continuous ethylene treatment was found to activate a protein kinase. This kinase also seemed to be induced by wounding. Full-length cDNAs of two Citrus mitogen-activated protein kinase genes (CMAPK1 and CMAPK2) were isolated from a Citrus cDNA library. Their recombinant proteins produced in bacteria were recognized by anti-human ERK antibodies, and the in-gel kinase activity assay showed that CMAPK2 recombinant protein was capable of autophosphorylation. Northern blot analysis showed that ethylene induced both genes transcriptionally. CMAPK2 was also induced by wounding treatment, whereas CMAPK1 was not. In situ hybridization revealed that both of the MAPK genes had unusual transcript distribution patterns across the fruit abscission zone. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
