ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
PCR-BASED MARKERS REVEAL GENETIC IDENTIY AND DIVERSITY IN SUBSET COLLECTIONS OF WILD AND CULTIVATED APPLE |
| Authors: | B. Vornam, K. Gebhardt |
| Keywords: | Malus, RAPD, cp-DNA, microsatellites, PCR |
| Abstract:
In order to assess genetic diversity of clones of a subset collection of wild apple which were collected at forest sites in the federal state of Hesse, PCR based methods were applied. RAPD-analysis allowed us to distinguish clones and single tree progenies. Two of five tested primer pairs, described as universal primers for angiosperms (Taberlet et al., 1991) can be used for the amplification of the cpDNA from both Malus species. Two microsatellite SSR-primer pairs (GD 96 and GD 162) described by Hokanson et al., (1998) generated amplification products with DNA of wild apple, tentative hybrid clones and a subset collection of Malus x domestica borkh, cultivars. In Malus sylvestris (L.) Mill, the size of the amplification products of the SSR-primers GD 9 ranged from 194 to 394 bp while GD 162 produced products from 234 to 400 bp. | |
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