ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
IDENTIFICATION OF CULTIVARS AND HYBRIDS IN SYRINGA AND CUCURBITA BY DNA FINGERPRINTING |
| Authors: | M. Pfosser, H. Yemelyanava, W. Leonhardt |
| Keywords: | Syringa spp., Cucurbita spp., AFLP, varietal identification, wide crosses, embryo rescue |
| Abstract:
We have tested the applicability of AFLP analysis in varietal identification in Syringa and parentage identification after hybrid breeding in Cucurbita in a practical micropropagation situation. Both in Syringa as well as in Cucurbita, AFLP fingerprints were highly reproducible and produced similar band patterns irrespective of the starting material being either tissue culture-derived or greenhouse material. A single primer combination yielded enough polymorphic bands to unequivocally discriminate among 13 closely related lilac cultivars. Following hybrid production after wide crosses between Cucurbita pepo x C. maxima lines AFLP fingerprints clearly identified the parental genomes in regenerating hybrid plants and could be used to discriminate between true hybrids and gynogenic haploids or spontaneous diploids. | |
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