ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
DIFFERENTIAL RAPD FINGERPRINTS IN CARROT TISSUES |
| Authors: | C. Schaefer, S. Schaffer, B. Arnholdt-Schmitt |
| Keywords: | ageing, Daucus carota, differential, kinetin, RAPD polymorphism, tissue specific |
| Abstract:
Genome fingerprinting by random amplified polymorphic DNA (RAPD) is a subject for critical discussion. Reproducibility of the method is often hampered by its high sensitivity to experimental and technical conditions and generation of a fingerprint pattern is highly determined by competition between primer binding sites in the genome of interest. Nevertheless, under standardized conditions, the especially high sensitivity of genome organisation to RAPD analysis may particularly offer the chance to reveal differential changes in genome organisation using markers. To test this hypothesis, RAPD fingerprints were performed on a primary culture and on different tissues and organs of carrot plants, including leaves of different ages. Polymorphic RAPD bands were discovered that were dependent on the regulation of cell division-growth, displayed tissue specificity or depended on the age of the tissue. The differential RAPD fingerprints were reproducible and occurred with different individual plants of similar genotypes. The observed polymorphism was obviously based on quantitative variation in the target genomes. Also, the putative loss of variable bands could be traced back to quantitative polymorphism by varying primer or template concentration. | |
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