ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
CHARACTERIZATION OF STEM QUALITY IN RELATION TO ROOTING PROCESS IN PRUNUS- AND AMELANCHIER- CULTURES |
| Author: | I. Pinker |
| Keywords: | Amelanchier lamarckii, Prunus kurilensis ‘Brillant’, shoot culture, micropropagation, histology of stem base, peroxidase activity, subculture duration, in vitro rooting |
| Abstract:
The interaction of stem quality and rooting was studied in easy-to-root and difficult-to-root shoot cultures. Stem properties and rooting performance were affected considerably by the duration of the last subculture on multiplication medium. Stem tissue matured during the extended last subculture and starch was accumulated. These processes were more pronounced in Prunus kurilensis ‘Brillant’ than in Amelanchier lamarckii. The initial peroxidase activity in the former was considerably higher than in the latter. In P. kurilensis rooting was accelerated by prolongation of the last subculture on multiplication medium from 4 to 7 weeks. In contrast, in A. lamarckii rooting potential decreased and root development was delayed if the last subculture lasted more than 5 weeks. | |
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