ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
THE EFFECT OF REDUCTION OF EXUDED PHENOLIC SUBSTANCES LEVEL ON ROOTING OF OAK MICROCUTTINGS |
| Authors: | J. Malá, J. Kálal, H. Cvrcková, M. Cvikrová, J. Eder |
| Keywords: | Oak micropropagation, polyvinylpyrolidone, lignification, acclimatization |
| Abstract:
In this study we have followed the influence of polyvinylpyrrolidone (PVP) on the development of root system of oak microcuttings (Quercus robur L.), changes in the contents of phenolic substances in roots, and in the culture media. The microcuttings from explants cultured in vitro for 2 years have been used for rooting. Originally, the explants were taken from 150 years old oak tree. For the induction of rooting on agar medium with high concentration of 1-  - naphthaleneacetic acid (NAA, 14.0 mg l-1) has been used.
After 1 week of etiolation, the microcuttings were displaced in light and transferred to agar media without NAA and with or without 1 % PVP. On the medium enriched with PVP 86.6 % of microcuttings rooted after 6 weeks.
No substantial differences in average rooting of control microcuttings, cultured without PVP, were found (81.5 %). The enhancement of the lenght of adventitious roots after 6 and 10 weeks of cultivation was observed in microcuttings cultured with PVP. Addition of PVP decreased the endogenous contents of phenolic substances in the roots (by about 20 %) as well as in the culture media after 10 weeks of cultivation.
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