ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
HOW TO PRODUCE LILY BULBLETS IN VITRO THAT PERFORM OPTIMALLY AFTER PLANTING |
| Authors: | M. Langens-Gerrits, G. de Klerk, A. Croes |
| Keywords: | Lilium ‘Star Gazer’, explant size, minerals, stem formation, sucrose |
| Abstract:
After planting in soil, micropropagated lily bulblets may sprout with a stem or with leaf-blade bearing scales. Bulblets with a stem grew much faster than bulblets with leaf-blade bearing scales. We studied which factors during tissue culture enhance stem formation. A regime of a long period (8–12 weeks) at high temperature (25 °C), followed by a short period (2–4 weeks) at moderate temperature (15 °C) proved to be essential. Large bulblets more often formed a stem than small bulblets. Culture conditions during the period at 15 °C had little effect on stem formation. Stem formation increased with increasing sucrose concentration in the medium, with increasing size of the explant from which the bulblets regenerated and with decreasing concentration of Murashige and Skoog-minerals. For all three factors we observed that increased stem formation coincided with low endogenous levels of phophorus and sulphur in the bulblets. | |
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