ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
ROOTING OF AXILLARY SHOOTS OF CODIAEUM VARIEGATUM BLUME CV. EXCELLENT OBTAINED IN VITRO FROM DEFOLIATED SHOOT EXPLANTS |
| Authors: | T. Orlikowska, I. Sabala, D. Kucharska |
| Keywords: | activated charcoal, gelling agent, gibberellic acid, IBA, mineral salt concentration |
| Abstract:
Shoots of Codiaeum variegatum 'Excellent' produced in vitro on defoliated explants were rooted on the MS medium with 1 mg l-1 indole-3-butyric acid (IBA). Almost all shoots, even the smallest one (5 mm long) rooted and doubled their length in the course of 4 weeks. In order to stimulate more shoot elongation during in vitro rooting, we decreased the IBA concentration to 0.5 mg l-1, excluded IBA from the medium with 3 g l-1 activated charcoal (AC) or supplemented AC medium with gibberellic acid (GA3). GA3 incresed shoot length insignificantly but also decreased the number of rooted shoots. Diluting the MS medium by half stimulated root growth when combined with 2 mg l-1 Gelrite instead of 7 g l-1 agar. | |
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