ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
IN VITRO PROPAGATION OF WILD CYCLAMEN PERSICUM MILL. FROM SEEDLING TISSUE |
| Authors: | A.M. Mohannad, N.S. Karam |
| Keywords: | cyclamen, micropropagation, organogenesis, BA, thidiazuron |
| Abstract:
Organogenesis of wild cyclamen was studied using in vitro seedling tuber, petiole, cotyledon, and root explants and MS media supplemented with 0.1 mg l-1 NAA and different concentrations of BA. The influence of BA, TDZ, or zeatin concentration on shoot proliferation was tested. Rooting of proliferated shoots was tried using different concentrations of NAA or IBA. The greatest percent shoot formation and number of shoots per explant were obtained from tuber explants cultured on media containing 2 mg l-1 BA. Root explants cultured on BA-free media exhibited the greatest root organogenesis. The greatest number of proliferated shoots per explant was obtained using 2 mg l-1 BA, 0.11 mg l-1 TDZ, or 1 mg l-1 zeatin. Incorporation of NAA or IBA at 0.5 or 1 mg l-1 into the media resulted in 86–100 % rooting. In vitro-rooted microshoots were successfully acclimatized. | |
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