ISHS Acta Horticulturae 530: International Symposium on Methods and Markers for Quality Assurance in Micropropagation
ADVENTITIOUS REGENERATION IN ROOT CULTURE OF A SELECTION OF PELARGONIUM X HORTORUM CULTIVARS: AN ASSESSMENT OF CAULOGENIC POTENTIAL AND GENETIC STABILITY |
| Authors: | B.M. Doyle, D.K. Lawton, A.C. Cassells |
| Keywords: | micropropagation, thidiazuron, TDZ, tri-iodobenzoic acid, TIBA |
| Abstract:
Root explants from twenty two Pelargonium x hortorum FI hybrids were assessed for their caulogenic potential in vitro in response to media containing 4.4 g l-1 Murashige and Skoog (1962) basal medium containing 30 g l-1 sucrose, 8 g l-1 agar and varying concentrations of the growth regulators tri-iodobenzoic acid (TIBA) and thidiazuron (TDZ) at pH 5.8. Caulogenic potential varied from zero to five shoots per explant. Twenty of the FI hybrids produced shoots. At least 50 rooted shoots from the control and root derived population for twenty genotypes were established in vivo and assessed visually for morphological uniformity up to flowering. Visual examination showed no signs of morphological variation with respect to leaf shape, petal shape, flower colour and plant habit except for cvs. Pink Elite and Red Elite. Progeny derived from the latter had 5.5 % and 10 % plants, with variable leaf shape, respectively. | |
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