ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
MONITORING GENETIC CHANGES OF AN APOMICTIC PHYLLOXERA SINGLE FOUNDER LINEAGE AS IT PROGRESSES TOWARDS AMPHIMIXIS |
| Authors: | A. Forneck, R. Blaich, M.A. Walker |
| Keywords: | Phylloxera, Dactulosphaira vitifoliae, single founder lineage, AFLP, genetic variation |
| Abstract:
Phylloxera (Daktulosphaira vitfoliae Fitch), a cyclical parthenogenetic aphid, is one of the most important pests in grapes. Cyclical parthenogenesis facilitates the production of the extensive numbers of offspring through thelytoky during the anholocyclical life stages and enhances genetic variation through amphimixis in the holocycle. A single founder lineage of phylloxera collected from 3309 C (Vitis riparia x v. rupestris) leaf galls in California was reared in an aseptic dual culture system on 3309 C, a moderately resistant rootstock. This system produced polyphenetic life stages, both asexual and sexual, that were genetically analysed using AFLP (amplified fragment length polymorphism) markers. Three primer combinations were utilized generating 93 bands of which four were polymorphic and indicated genetic diversity within apomictically reproducing phylloxera lineages. This work presents the initial experiment studying genetic variation in reproducing phylloxera lineages that approach amphiximis and investigates potential genetic variability of this pest. | |
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