ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
MORPHOLOGICAL RESPONSES OF MUTANTS FROM GRAPEVINE ROOTSTOCK CV. GRAVESAC BY IN VITRO MICROGRAFTINGS |
| Authors: | A. Lima Da Silva, P. Hariscain, N. Ollat, J.P. Doazan |
| Keywords: | Stem length, leaf area, biomass, vigour, mutation, grapevine, rootstock |
| Abstract:
A behaviour study of mutants of the grapevine variety Gravesac (5306, 5327, 5338 and 5339), issued from a 30 Gy dosis of Cs137 irradiation, was carried out with the aim to characterize the development of different "mutant/Gravesac" reciprocal graft combinations as compared to the self-grafted control and to try and locate the major mutation site. Whether the mutant is rootstock or scion greatly influences the combination vigour, e.g. when the mutant is a rootstock, the combination development presents a significant reduction for all the parameters. On the contrary, when it is a scion, the behaviour varies in relation with the self-grafted control. When comparing each association of the same mutant, we noted that 5306 and 5339, as rootstocks, led to a decrease in scion development (Gravesac) compared to when they are in a scion position. On the contrary, 5338 conferred a higher vigour. For the mutant 5327, these characteristics remained stable. However, in spite of a grafting effect which led to a growth decrease in the self-grafted control, we observed a development increase (dry weight and leaf area) of the combinations, except for 5338 as scion, as compared to the same mutants ungrafted. But, when comparing dry weights from all the graft combinations, both direct and reciprocal, leaf and stem biomass (conferred vigour) tended to lessen if the mutants were used as rootstocks. We also followed dry matter distribution between aerial and root parts of the different combinations. All these results helped us to locate the major mutation site. For instance, 5338 was affected mainly in the aerial part. For 5339, the mutation affected the root part. For 5306 and 5327, mutation effects were more complex and indicated that both aerial and root parts were affected. Besides, the micrografting technique can be used to measure vigour variations between the different combinations. It opens up to research on conferred vigour and in vitro selection. | |
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