ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
PHOTOSYNTHESIS, DARK RESPIRATION AND TRANSPIRATION OF IN VITRO CULTURED VINE PLANTS DURING THEIR ACCLIMATIZATION |
| Authors: | T. Slavtcheva, V. Dimitrova |
| Keywords: | Grapevine (V. vinifera L.), gas exchange, lighting, temperature, variety, in vitro culture mode |
| Abstract:
Investigations were carried out on photosynthesis, dark respiration and transpiration of in vitro cultured Dimiat (clone candidates - 4/24 and 4/38) and Riesling Italian (clone candidate - 3/47) vine plants during their acclimatization period. There were in the trial two groups of plantlets: obtained with explants from field-grown vines i.e. initially in vitro propagated (IP); micropropagated of 17th, 15th, 15th subcultures respectively (MP). Gas exchange was investigated by means of appropriate methods specially elaborated. Rates of photosynthesis and dark respiration were measured with infrared gas analyzer two and four weeks after transfer from in vitro culture. Transpiration rates were determined after weight methods. The photosynthetic rates were measured under four different lightings: 1000, 5000, 10000 and 20000 lx. Results showed that the photosynthesis of in vitro plants was strongly affected by the light intensity. Considerable differences in the gas exchange were established according to air temperatures (21°C and 26°C), as well. Rates of photosynthesis, dark respiration, and transpiration decreased generally in the course of time after transfer from in vitro culture. Gas exchange differences were found also according to the other factors studied (vine variety, mode of in vitro culture), but they were, however, most frequently non- significant. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
