ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
PROTECTION AGAINST GRAPEVINE FANLEAF VIRUS IN TRANSGENIC TOBACCO CONTAINING NON-TRANSLATABLE SEQUENCES |
| Authors: | C. Monier, P. Barbier, B. Walter |
| Keywords: | Court-noué disease, Nicotiana benthamiana, resistance to disease, immunity |
| Abstract:
Fanleaf degeneration, caused by two nepoviruses (GFLV, grapevine fanleaf virus and ArMV, arabis mosaic virus) is one of the most devastating viral diseases of grapevine. No natural resistance has been discovered yet. In order to obtain plants resistant to GFLV and/or ArMV, different strategies based on genetically engineered pathogen-derived resistance have been investigated. Three chimaeric genes derived from the sequence of GFLV (strain F13) were constructed under the control of a constitutive promoter (CaMV 35S2): (1) the coat protein (CP) gene made non translatable; (2) this CP gene joined with the downstream 3' non translated part of the GFLV RNA2; (3) this truncated 3' non translated part, duplicated. These constructs were used to transform Nicotiana benthamiana and the 110 Richter (Vitis Berlandieri x V. rupestris) grapevine rootstock. Evidence is presented of a protection of transgenic N. benthamiana for each of the three constructs. | |
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