ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
RESISTANCE TO NEPOVIRUSES IN GRAPEVINE AND NICOTIANA BENTHAMIANA: EXPRESSION OF SEVERAL PUTATIVE RESISTANCE GENES IN TRANSGENIC PLANTS |
| Authors: | A. Spielmann, V. Douet-Orhant, P. Gugerli, S. Krastanova |
| Keywords: | coat protein-mediated virus resistance, nepovirus, Vitis rupestris, Nicotiana benthamiana |
| Abstract:
Agrobacterium tumefaciens strain LBA 4404 harbouring a disarmed binary vector which contained chimeric genes for kanamycin resistance and the coat protein (CP) of the arabis mosaic nepovirus (ArMV) or the grapevine fanleaf nepovirus (GFLV) were used in co-cultivation experiments with leaf discs of Nicotiana benthamiana or somatic embryos of the grapevine cultivar Vitis rupestris. Transgenic N. benthamiana or grapevine plants expressing the ArMV-CP or the GFLV-CP genes were regenerated and accumulation of the transgenic CP analysed by ELISA. Whereas GFLV-CP and ArMV-CP accumulation could be demonstrated in transgenic N. benthamiana plants, no transgenic CP could be detected in grapevine tissues. Furthermore, expression of the ArMV-CP in N. benthamiana resulted in the formation of virion-like particles, as revealed by electron microscopy studies. Both GFLV-CP and ArMV-CP expressing transgenic N. benthamiana lines showed resistance against GFLV or ArMV, respectively, whereas no protection could be demonstrated for the transgenic grapevine plants. | |
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