ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
COMPARATIVE IN VITRO DEVELOPMENT OF FIVE GRAPEVINE ROOTSTOCK VARIETIES AND MUTANTS FROM THE CULTIVAR 'GRAVESAC' |
| Authors: | A. Lima da Silva, P. Hariscain, N. Ollat, J.P. Doazan |
| Keywords: | development, morphological measurements, in vitro selection, vigour |
| Abstract:
The behaviour study of 5 grapevine rootstock varieties of distinct genetic origin (SO4, 140 Ru, Riparia, Fercal and Gravesac) has underscored significant differences mainly concerning shoot and root development and number. A strong correlation was found between in vitro plant fresh weight and dry weight, and between stem length, leaf area and total dry biomass. Concerning leaf and root number, as well as root length, no significant link was found with other parameters. In the case of the Gravesac mutants, significant reductions in development were observed when compared to the control, concerning stem length, root elongation and particularly leaf area. A strong correlation was also found between stem length, dry weight and leaf area. The decrease in dry matter seems to be linked mainly to a decrease in leaf development. The characteristics of a vigour decrease in the mutants are passed on and remain stable through in vitro vegetative propagation. Such results confirm the observations made between different rootstocks and lead to the statement that stem length, leaf area and dry weight are a good indication of grapevine development in vitro. These parameters are probably the most useful for early selection of mutants for vigour. We checked particularly the correlation of in vitro stem length with the same genotypes growth during their initial acclimatization period in the greenhouse. | |
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