ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
GENETIC TRANSFORMATION OF TOBACCO AND GRAPEVINE FOR RESISTANCE TO VIRUSES RELATED TO THE RUGOSE WOOD DISEASE COMPLEX |
| Authors: | L. Martinelli, D. Costa, V. Poletti, S. Festi, N. Buzkan, A. Minafra, P. Saldarelli, G.P. Martelli, A. Perl |
| Keywords: | genetic transformation, movement protein, rugose wood disease complex, Vitis |
| Abstract:
In the framework of a study aiming at producing transgenic grapevines resistant to viruses of the rugose wood disease complex, the movement protein (MP) genes of Grapevine virus A (GVA) and B (GVB) were analysed in transgenic Nicotiana plants. The cassettes transcribing MP genes in their sense (MP+) and antisense (MP-) orientation were transferred to Nicotiana benthamiana and N. occidentalis by Agrobacterium tumefaciens-mediated transformation. Regenerants expressed the RNA transcripts. R1 seedlings expressing the antisense gene, when challenge-inoculated with the homologous virus showed a consistent reduction of virus titer, and did not show symptoms up to 18 days post inoculation. MP+ lines showed lower ELISA readings and percentage of symptomatic plants as compared with the non-transgenic control. Both constructs were successfully inserted in somatic embryos of Vitis rupestris and V. vinifera cv. Superior seedless, from which several plants were regenerated. | |
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