ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
ANALYSIS OF 41 B (VITIS VINIFERA X VITIS BERLANDIERI) GRAPEVINE ROOTSTOCKS FOR GRAPEVINE FANLEAF VIRUS RESISTANCE |
| Authors: | M.C. Mauro, P. Coutos-Thevenot, M. Boulay, L. Valat, P. Barbier, B. Walter, L. Pinck |
| Keywords: | Virus, Vitis, transgenic, resistance, micro-grafting, protoplast, nematode |
| Abstract:
Grapevine fanleaf virus (GFLV) is a nepovirus causing important damages in French vineyards and all over the world. The chemicals used to control fanleaf degeneration are applied in the soil to kill the nematodes vectors of the virus. These nematicides will be probably prohibited in all vineyards in a few years to limit pollution of ground water. The use of transgenic plants resistant to GFLV by integration of a viral gene is a strategy useful to develop. Three genes, coat protein (CP), RNA polymerase (replicase) and proteinase gene have been cloned and transferred via Agrobacterium tumefaciens into 41B grapevine cells. Expression of these genes in the regenerated plants was detected through Elisa test and Northern blot analysis. In order to evaluate resistance of the transgenic plants, challenge with GFLV was performed using micrografting and electroporation of virus into grapevine protoplasts. The protocols were set up. So far for the coat protein strategy no clear-cut virus resistance efficiency was obtained when comparing the results of the different tests. For the defective replicase gene and for non-translatable CP gene promising results were obtained from tobacco inoculation experiments. These constructs have been introduced into 41B plants. Bioassays conducted for these plants are underway. Experimental plots have been also set up in the vineyard to assess conformity of the transgenic 41B rootstocks, virus tolerance and possible biorisks associated with the use of woody perennial crops. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
