ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
REGENERATION AND TRANSFORMATION OF DIFFERENT EXPLANTS OF VITIS VINIFERA SPP. |
| Authors: | M. Harst, B.-A. Bornhoff, E. Zyprian, R. Töpfer, G. Jach |
| Keywords: | Agrobacterium, Vitis vinifera, somatic embryogenesis, suspension culture, transformation |
| Abstract:
Embryos originating from anther cultures of Vitis vinifera cv. DORNFELDER which were either cultured on solid or in liquid medium were cocultivated with Agrobacterium tumefaciens strain LBA 4404 harbouring a plasmid with ß-glucuronidase and neomycinphosphotransferase II as the selectable marker gene. Best conversion rates of selected shoots to intact plants could be obtained when embryos of suspension cultures were used for gene transfer purposes. The transformation protocol established for DORNFELDER could be transferred to the most important white vine variety of Germany, RIESLING, using two different plasmids carrying anti-fungal gene constructs. The transgenic DORNFELDER and RIESLING plantlets could be successfully adapted to glasshouse conditions. | |
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