ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
EVALUATION OF GENETIC DIVERSITY OF GRAPEVINE CULTIVARS GROWN IN MOROCCO WITH RAPD-PCR |
| Authors: | S. Faraj, J.L. Cenis, E. Sánchez-Escribano, J.M. Ortiz |
| Keywords: | Identification, molecular markers, table grapes |
| Abstract:
Forty five accessions of grapevine germplasm were studied by using the random amplified polymorphic DNA (RAPD) technique for identification. Autochthonous plus foreign varieties were included in the study. All the accessions are included in the grapevine collection located at Mèsnes (Morocco). Twelve primers were chosen for the analysis based on the obtained polymorphism. A presence/absence matrix was prepared with the polymorphic bands, and a dendrogram was obtained from it with the UPGMA method. Results indicate that some of the studied varieties are synonyms, while some of the others have a high similarity, although further studies are needed in order to confirm the existence of a synonymy. Relationships among the studied varieties are discussed, mainly with reference to the autochthonous cultivars. Detection of a high variability within the autochthonous material is evidenced. As a consequence of the high level of polymorphism obtained, all the studied accessions could be identified. The use of the RAPD technique for identification of grapevine material is recommended. | |
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