ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
FINGERPRINTING OF GRAPE CULTIVARS GROWN IN CHILE : COMPARISON OF METHODS BASED ON ANONYMOUS SEQUENCES AND A SET OF MICROSATELLITE LOCI |
| Authors: | P. Hinrichsen, C. Narváez, J. Valenzuela, C. Muñoz, J.E. Bowers, C.P. Meredith |
| Keywords: | RAPD, AFLP, SSR, STMS, Vitis vinifera, Vitis labrusca |
| Abstract:
Considering the high heterozygosity index of grapes, microsatellites (SSRs), codominant markers that have become widely used for fingerprinting, are powerful tools to characterize and differentiate cultivars. In this work, a panel of 11 SSR markers (STMS) was used to characterize a collection of 57 genotypes (49 cultivars) maintained at La Platina Experimental Station (Chile), representing wine and table grapes of different skin colours. This approach was compared to RAPD and AFLP methods, both considered dominant-type markers that rely on the analysis of random PCR-amplified genomic sequences. All genotypes were clearly differentiated with the entire set of 11 SSRs. A total of 88 different alleles were identified in this population. In the case of the anonymous markers RAPD and AFLP, 18 RAPD primers rendered 103 polymorphisms and four AFLP combinations generated 86 polymorphic bands (5.7 and 21.5 informative bands per reaction, respectively), illustrating the potential of both approaches for grapevine fingerprinting. These results suggest that both random-primer methods could be used in parallel to SSR for genetic identification purposes. Moreover, when parameters representing the informativeness of each marker system were calculated, the balance strongly favored the use of RAPD or AFLP vs. SSRs. However, if these analyses include factors like reproducibility of the results among laboratories or technical difficulties of the assay, the use of SSR appears more favorable. | |
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