ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
DIVERSITY ASSESSMENT OF SEEDLINGS FROM SELF-POLLINATED ‘SANGIOVESE’ GRAPEVINES (VITIS VINIFERA L.) BY AMPELOGRAPHY AND MICROSATELLITE DNA ANALYSIS |
| Authors: | I. Filippetti, C. Intrieri, M.R. Thomas, O. Silvestroni |
| Keywords: | Vitis vinifera, ampelography, microsatellite, cultivar, clone |
| Abstract:
A population of V. vinifera L. seedlings deriving from a single self-pollinated ‘Sangiovese’ vine was assayed for diversity by ampelographic and genetic techniques. After field-transplant in 1987, the seedlings were initially screened in 1995, the results showing that 24 were of standard vigour and grape production. Woody cuttings from the 24 seedlings and the mother plant were self-rooted in 1995, and each vine was morphologically analysed and compared in 1997 using 31 descriptors from the OIV ampelographic data sheet (1983) which are also recommended by UPOV for varietal identification - 3 for young shoots, 7 for shoots in bloom, 17 for adult leaves, 1 for flowers and 3 for berries. DNA was extracted in 1996 from young apical leaves of the mother plant and the 24 selected offspring. Ten molecular microsatellites, VVS1, VVS2, VVS3, VVS16, VVS29, VVMD5, VVMD6, VVMD7, VVMD17 and VVMD28, were used for progeny and mother plant comparison. The descriptor-based analysis showed that twelve of the 24 seedlings were morphologically similar amongst themselves and not different from the mother; the remaining twelve differing from each other and from the parent. The microsatellite analysis differentiated all 24 seedlings from the mother plant. Only two seedlings showed the same allele patterns at the ten tested loci, although they differed morphologically. Taken together, the results of both analyses indicate that self- pollination can generate phenotypically similar individuals that are difficult to distinguish morphologically, while their genetic polymorphism can easily be detected by microsatellite analysis. It is thus possible, as suggested by Rives (1961), that certain old cultivars comprise a number of clones that derive via vegetative propagation from closely related mother plants. Corroborating the polyclonal origin in such cases can 1be performed by techniques combining morphological and molecular approaches. | |
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