ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
FINGERPRINTING OF THE MAIN VITIS VINIFERA VARIETIES GROWN IN THE NORTHERN REGION OF PORTUGAL |
| Authors: | F. Ferreira Monteiro, E. Nunes, R. Magalhaes, M.A. Faria, J.E. Bowers, C.P. Meredith, A. Martins |
| Keywords: | Microsatellites, characterization, identification, cultivar, PCR, vines |
| Abstract:
The methods based on phenotypic characteristics commonly used for Vitis vinifera variety identification do not always give the most accurate information because of the interaction of the genotype with the environment. The genetic based methods overcome this situation. We have been developing a DNA fingerprinting method to characterize and clarify the identity of grapevine varieties studied. For this purpose we used thirty-one varieties of Vitis vinifera from the Northern region of Portugal and three Spanish varieties that could possibly be the same as three of the Portuguese varieties. Within a wide range of molecular markers we used a method based on microsatellites. This work included all steps of characterization from sampling through fingerprinting. The microsatellites were amplified with six primers using PCR techniques. From the amplification, we obtained DNA, which by denaturation and PAGE formed one or two bands of different sizes that constitute part of the fingerprinting. The use of silica gel to preserve the samples and the DNA extraction from dried leaves proved to be a very efficient method. The microsatellites were amplified with 6 primers using PCR techniques. The cluster analysis detected possible synonym problems, indicating that some varieties could be the same even if they show different names. The analysis also showed three main agglomeration groups within the tested varieties. | |
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