ISHS Acta Horticulturae 528: VII International Symposium on Grapevine Genetics and Breeding
BROAD RANGE GENOTYPING USING MICROSATELLITE MARKERS IDENTIFIED IN VITIS RIPARIA |
| Authors: | K.M. Sefc, J. Glöβl, H. Steinkellner, F. Regner |
| Keywords: | Vitis spp, microsatellites, genetic analysis |
| Abstract:
We identified 18 polymorphic microsatellite (SSR) markers from a genomic library of Vitis riparia. Heterologous amplification of the loci in 7 related Vitis species (including Vitis vinifera) and in interspecific hybrids was successful in most cases. Therefore, these markers are applicable to genotyping of a range of Vitis species. With the 12 most suitable markers for grapevines, the probability to obtain identical SSR profiles from two unrelated individuals is 6.8 x 10-10. We genotyped over 100 cultivars using 10 SSR markers and were able to detect a unique genotype for all but color variants. The same degree of discrimination was obtained when the five most polymorphic markers were used, indicating the intense information content of this type of markers. 30 rootstock cultivars were investigated, whre only clones, as SO4 and Binova, could not be discriminated. Comparing the size range of alleles between rootstocks and grapevines, rootstock specific alleles were detected at several loci. To further characterize color types of Pinot (Pinot blanc, Pinot noir and Pinot gris) that were not distinguishable with 25 microsatellite markers we applied AFLP technique. About 3000 bands were scored, but except differences between the intensities of corresponding bands, no clear differentiation was possible. DNA extraction protocols to genotype grape berries and raisins have been worked out. DNA was obtained from both sources, but it was highly degraded in the case of raisins. The suitability of the DNA for PCR amplification of sing le genetic loci was shown by implification of 11 microsatellite markers. | |
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