ISHS Acta Horticulturae 526: V International Symposium on Grapevine Physiology
BETA-1,3-GLUCANASE MRNA SYNTHESIS IN GRAPEVINE LEAVES AFTER BOTRYTIS INOCULATION, AND IN BERRIES AFTER WOUNDING OR DURING RIPENING |
| Authors: | E. Kraeva, A. Renault, N. Terrier, C. Tesnière, C. Romieu, F. Sauvage, J. Bierne, A. Deloire |
| Keywords: | Beta-1,3-glucanase, pathogenesis-related proteins, development, leaves, berries, grapevine |
| Abstract:
The hydrolytic enzymes beta-1,3-glucanases (EC 3.2.1.39) are known to be involved in plant defense reactions against pathogens or in developmental events. Transcription of beta-1,3-glucanase gene was studied in grapevine (Vitis vinifera L.): in leaves after induction with Botrytis cinerea, or in berries after mechanical wounding; and during berry development. Northern blot hybridization showed that beta-1,3-glucanase mRNA strongly accumulated in grapevine leaves following infection with Botrytis. The transcript was detectable at 2 days after infection, and reached a maximum level at 4 days post-infection. During berries development, no constitutive expression was observed during their green growth period (before ripening), whereas the synthesis of  -1,3-glucanase was detected during fruit ripening.
On the contrary, mRNA of this isoform was induced over fruit green growth period in response to wounding treatments.
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