ISHS Acta Horticulturae 521: XXV International Horticultural Congress, Part 11: Application of Biotechnology and Molecular Biology and Breeding - Gene Expression and Molecular Breeding, Genome Analysis.
PRODUCTION AND GENE ANALYSES OF ALIEN MONOSOMIC ADDITION LINES OF ALLIUM FISTULOSUM L. WITH EXTRA CHROMOSOMES FROM WILD SPECIES IN SECTION CEPA OF ALLIUM |
| Authors: | Y. Tashiro, M. Tsutsumi, M. Shigyo |
| Keywords: | Allium, Wild species, Alien monosomic addition line |
| Abstract:
To produce the alien monosomic addition lines of Allium fistulosum (2n=16, FF) with extra chromosomes from three wild species, A. galathum (GG), A. oschaninii (OO), and A. vavilovii (VV), reciprocal crossings were carried out between triploid hybrids (2n=21˜24, FFG, FFO, FFV) and A. fistulosum. Chromosome numbers of the seedlings ranged from 16 to 24. The aneuploid seedlings (2n=18 – 23) were backcrossed to A. fistulosum again. Karyotypic and meiotic analyses of the seedlings revealed that 23 plants of alien monosomic addition lines were produced. The extra chromosomes of 18 out of the 23 plants were identified: the one plant, FF+6G; one plant, FF+7O; seven plants, FF+4V; and nine plants, FF+ 7V. Moreover, isozyme, 5S ribosomal DNA, and RAPD markers were established between the three wild species and A. fistulosum. Then, these markers were examined in the 18 plants with identified extra chromosomes. All the 18 plants showed isozyme and/ or 5S ribosomal DNA markers specific to the chromosome donors: FF+6G, Adh-1 and Got-2; FF+7O, 5S-Rdna-04; FF+4V, Mdh-1 and Pgm-1; and FF+7V, 5S-Rdna-V2. Several RAPD markers of chromosome donors were also detected in these plants. Thus, chromosomal locations of some isozyme genes and DNA markers of the three wild species were determined in this study. | |
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