ISHS Acta Horticulturae 521: XXV International Horticultural Congress, Part 11: Application of Biotechnology and Molecular Biology and Breeding - Gene Expression and Molecular Breeding, Genome Analysis.
CDNA CATALOGS EXPRESSED IN ALBEDO OF CITRUS FRUIT: A COMPARATIVE ANALYSIS OF CDNA LIBRARIES FROM PULP AND ALBEDO OF SATSUMA MANDARIN (CITRUS UNSHIU MARC.) |
| Authors: | K. Masayuki, T. Endo-Inagaki, T. Moriguchi, M. Omura |
| Keywords: | Citrus unshiu, albedo, cDNA library, random sequencing, redundancy |
| Abstract:
As an important part of Citrus genome analysis in Japan, we have been cataloguing cDNA clones expressed in Satsuma mandarin (Citrus unshiu Marc.) fruit. The cDNA library was constructed from albedo at the matured stage and used for random sequencing analysis. Among 446 clones successfully analyzed, 214 clones (47.9%) showed significant homology to genes which had been registered in the DNA database. It included metabolism-related enzymes such as ketolase, aldolase, glyceraldehyde 3 phosphate dehydrogenase, UDP-glucose pyrophosphorylase. A wide variety of protein kinases and phosphatases which were assumed as signal transduction- and cell cycle-related proteins were also identified. Possible cell growth- and expansion-related genes were also detected. The clones homologous only to expressed sequence tags (ESTs), whose functions were not clear, were taking account for 29.1% (130 clones) and the remaining clones did not provide any specific information from the database. Among these clones, only two genes were expressed redundantly. The gene repertories and redundancy of cDNAs from albedo were analyzed to characterize the physiological status on the molecular bases in citrus fruit at the maturation stage in comparison to previous analyses. | |
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