ISHS Acta Horticulturae 520: XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding - In Vitro Culture.
INDUCTION AND CONVERSION OF SOMATIC EMBRYOGENESIS ON THE ANTHER FILAMENT OF SPATHIPHYLLUM SCHOTT |
| Authors: | S.P.O. Werbrouck, T.G.R. Eeckhaut, P.C. Debergh |
| Keywords: | Spathiphyllum, Araceae, conversion, embryogenesis |
| Abstract:
A prerequisite for genetic manipulation, mutation breeding or ploidy manipulation of the ornamental crop Spathiphyllum is the availability of a system which allows a target cell to regenerate into a new plant. Induction of somatic embryos could offer a powerful tool to reach this goal. When placed on a Murashige & Skoog medium supplemented with 10 μM naphthaleneacetic acid (NAA) and 0.2– 3 μM thidiazuron (TDZ), anthers of different genotypes develop clumps of attached somatic embryos on the filament. The younger the anthers, the more sensitive their filament cells were for the induction of somatic embryogenesis. After six weeks, the somatic embryos were transferred to a Murashige & Skoog (MS) medium supplemented with 10 μM benzylaminopurine (BA) + 0.02 μM NAA to develop secondary somatic embryos. Another 6 weeks later, they were ready for conversion. The best conversion medium was a hormone-free MS medium. Addition of abscisic acid (ABA) had a negative effect. After a conversion period of 10 weeks, the normal looking plants were transferred to the greenhouse. | |
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