ISHS Acta Horticulturae 520: XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding - In Vitro Culture.
SEASONAL DIFFERENCES IN EX VITRO GROWTH AND CORM FORMATION BETWEEN TWO MICROPROPAGATED SAGITTARIA LATIFOLIA ECOTYPES |
| Authors: | M.E. Kane, M.R. Gillis, N. Philman, S. Campbell |
| Keywords: | Sagittaria latifolia, corms, ecotypes, habitat restoration, micropropagation, photoperiod, wetland plant |
| Abstract:
Commercial micropropagation of wetland plants used for habitat restoration provides an alternative to field collection that could facilitate selection, rapid production, and storage of ecotypes that are genetically adapted to specific habitat conditions. Limited information is available concerning the degree of ecotypic variation within and between wetland plant populations and the relevance to successful habitat creation or restoration. Ecotypic differences in seasonal responses of growth and corm formation following acclimatization, were examined using micropropagated Sagittaria latifolia Willd. (Duck-potato), a highly variable herbaceous wetland species that is widely distributed in southeastern Canada and the eastern United States. Plants obtained from populations in Rhode Island and South Carolina were established in vitro from surfaced-sterilized rhizome shoot-tips and multiplied on agar-solidified medium consisting of half-strength Murashige and Skoog mineral salts, 0.56 mM myo-inositol and 1.2 μM thiamine supplemented with 87.6 mM sucrose and 1.1 μM benzyladenine. At 28-day intervals for 2 years, Stage II microcuttings of each ecotype were acclimatized and maintained under prevailing environmental conditions in north central Florida. Plantlet number, height and corm number and dry weight were determined 42 days post-transplantation. Significant seasonal differences in plantlet production, height and corm formation were observed between the ecotypes. The ecotypes exhibited differences in timing and duration of plantlet and corm formation. During the growing season, induction of corm formation occurred progressively earlier in the more northern Rhode Island ecotype. A differential latitudinal response to photoperiod is suggested. | |
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