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ISHS Acta Horticulturae 520: XXV International Horticultural Congress, Part 10: Application of Biotechnology and Molecular Biology and Breeding - In Vitro Culture.

BUD SCALE MATURATION IN SASKATOON BERRY (AMELANCHIER ALNIFOLIA NUTT.) PLANTLETS FOLLOWING IN VITRO HORMONAL TREATMENTS

Authors:   Brian D. Baldwin, M. S. Bandara, Karen K. Tanino
Keywords:   Dormancy, bud maturation, abscisic acid, benzylaminopurine, tissue culture
Abstract:
Bud scale maturation represents one of the early steps in endodormancy induction. Since abscisic acid (ABA) has been associated with dormancy, the role of this and other phytohormones in the maturation of bud scales was examined. Maturation of bud scales was achieved in vitro in saskatoon berry (Amelanchier alnifolia Nutt.) plantlets with a combination of 50 μM ABA and 5.5 μM 6-benzylaminopurine (BAP). Abscisic acid alone did not induce bud scale maturation but the greatest inhibition of bud break occurred under ABA treatments despite the addition of growth-promoting phytohormones. Unlike BAP, alpha-naphthaleneacetic acid (NAA) was ineffective in counteracting growth inhibition due to ABA treatments. The absence of NAA, however, was a requirement for bud scale maturation.

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