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ISHS Acta Horticulturae 516: XXV International Horticultural Congress, Part 6: Culture Techniques with Special Emphasis on Environmental Implications Physiological Processes in Plants

SEASONAL VARIATIONS OF THE IRIDOIDS, FLAVONOIDS AND VERBASCOSIDE CONTENT FROM VERBENA OFFICINALIS L

Authors:   M.I. Calvo, A. Crespo, M. Fernández
Keywords:   Verbena officinalis, Verbenaceae, experimental field, flavonoids, HPLC, iridoids, verbascoside, verbenalin
Abstract:
The aim of this work is the quantitative determinations of iridoids, flavonoids and verbascoside by HPLC in leaves of Verbena officinalis during three vegetative periods. The effects of plant age, date of harvest, type of soil, climate, as well as a comparision between two different locations, Ilundain and Lezaun, are been investigated in order to evaluate the development and content of major compounds in Verbena leaves, verbenalin mainly, because it is the active principle.

The highest amount of iridoids and flavonoids was found in the leaves during the pre-flowering period. After flowering the total compound content in the leaves decreases, whereas verbascoside content increases. The amount of iridoids and phenolic components are increasing by the years. Verbenalin content is greater in Lezaun than in Ilundain every vegetative cycle.

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