ISHS Acta Horticulturae 514: XXV International Horticultural Congress, Part 4: Culture Techniques with Special Emphasis on Environmental Implications
CARBON AND NITROGEN STATUS OF FLOWER-INDUCED STRAWBERRY AS REVEALED BY 13C- AND 15N-TRACER STUDIES |
| Authors: | A. Yamasaki, T. Yoneyama, F. Tanaka, K. Tanaka |
| Keywords: | 13C, 15N, allocation, flower initiation, strawberry, tracer |
| Abstract:
The involvement of carbon and nitrogen allocation and metabolism in the flowering process of strawberry was investigated by using 13C- and 15N-tracers. Strawberry (cv. 'Toyonoka') plants were grown under flower-inductive conditions (low temperature + short photoperiod) or non-inductive conditions (high temperature + long photoperiod). On the 15th day, 15N was applied as a solution of KNO3. 13C-labelled carbon dioxide (13CO2) was fed at a constant concentration for 6 hours in an assimilation chamber on the 17th day, when the flower buds were induced under flower-inductive conditions. Plant samples were harvested immediately after and 24 hours after 13C feeding. The 13C and 15N abundances were analyzed with an ANCA-SL mass spectrometer. In flower-induced plants, slightly more C was allocated to roots, and less C to young leaves and petioles than in non-induced plants. In contrast, more N was allocated to shoot apices, crowns and roots of induced plants than those of non-induced plants. From a central part of a crown, 1.8mm-thick sections were cut in vertical direction to analyze the location of contents and allocation of 13C and 15N within a crown. In the crown of flower-induced plants, more 15N was allocated to the shoot apex and the upper part of the crown, while the allocation gradient of C was not affected by the treatments. These results suggest stimulation of N metabolism in shoot apex and crown in induced plants and little contribution of recently assimilated carbohydrates to the flower induction process. | |
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