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ISHS Acta Horticulturae 510: VII Eucarpia Meeting on Cucurbit Genetics and Breeding

POSSIBILITIES OF INDUCING POLYEMBRYONY IN CUCUMBER (CUCUMIS SATIVUS)

Authors:   P. Nowaczyk, L. Nowaczyk
Keywords:   2,4D, benzylaminopurine, cucumber, fertility, growth regulators, polyembryony
Abstract:
Growth regulator influence on cucumber (Cucumis sativus) fruit fertility and the possibility of inducing polyembryony were observed for two cultivars, Polan F1 and Iwa. Fruit fertility was considered as the number of seeds collected from a fruit. The growth regulator 2,4 dichlorophenoxyacetic acid (2,4D) was applied alone or in combination with benzylaminopurine (BAP) in 0.0025% to 0.01% water solutions by plunging pollinated flowers into the solutions. Some of the flowers had been hand-pollinated and others had been open-pollinated by insects. Hand-pollination without growth-regulator application served as the control treatment. Experiment efficacy, expressed as the number of fruit obtained per number of flowers treated, ranged from 8% to 30%. For both cultivars, a lower level of fruit fertility and germinability were observed in fruit derived from flowers treated with 0.01% 2,4D. Among all germinating seeds, only one (from ‘Iwa’), hand-pollinated and treated with 0.01% 2,4D + 0.01% of BAP, was bi-embryonal. Both of the twin plants were diploids.

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