ISHS Acta Horticulturae 510: VII Eucarpia Meeting on Cucurbit Genetics and Breeding
MOLECULAR AND PHENOTYPIC VARIATION IN MELON PI 313970 |
| Author: | James D. McCreight |
| Keywords: | Cucumis melo, germplasm, lettuce infectious yellows virus (LIYV), PCR, RAPD |
| Abstract:
Melon plant introduction (PI) 313970 is a potential source of resistance to lettuce infectious yellows virus (LIYV). PI 315410 has the same origin as PI 313970. All tests for virus resistance were done using progenies 90625 and 33518, which had been derived by self-pollination of three or four plants of PI 313970. In two greenhouse plantings, PI 313970, PI 315410 and 33518 exhibited similar variation for growth habit, vigor, and gross morphological appearance (plant size, chlorosis, and flowering habit). PI 313970 and PI 315410 exhibited similar variation for fruit skin pattern, whereas 33518 had no obvious fruit skin pattern. Significant differences were found for length and width of cotyledons, and pistillate flower diameter, but not for hypocotyl length or staminate flower diameter. They were indistinguishable for internode, leaf, and floral trichome characteristics. Eleven RAPD primers revealed 74 monomorphic bands and 7 polymorphic bands. PI 315410 and 33518 were similar for five of seven polymorphic RAPD bands. The different and less variable rind color and pattern exhibited by 33518 fruits would be expected by chance alone. It remains to be demonstrated whether PI 313970, PI 315410 and 33518 are similar in their response to infection by LIYV. | |
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