ISHS Acta Horticulturae 509: VI International Symposium on Mango
ETIOLOGY OF MANGO MALFORMATION DISEASE USING GUS TRANSFORMANTS OF FUSARIUM SUBGLUTINANS |
| Authors: | S. Freeman, M. Maimon, Y. Pinkas |
| Keywords: | F. moniliforme, Fusarium spp., Fusarium subglutinans, Mangifera indica L., β-glucuronidase |
| Abstract:
Fusarium subglutinans has been associated with mango floral and vegetative malformation although confusion still remains regarding the etiology of the disease. A wildtype isolate of F. subglutinans, causing mango malformation disease, was transformed with the GUS (  -glucuronidase) reporter and hygromycin resistance genes.
Five stable transformants were isolated containing varying copy numbers at different integration sites.
Specific GUS activity was quantified for the transformants whereas no activity was recorded for the wildtype isolate.
The transformants and wildtype isolate were inoculated into healthy mango floral and vegetative buds.
Typical symptoms of misshapen shoots with short internodes, stubby leaves and bunchy, malformed inflorescences were observed 6 to 8 weeks following inoculation.
The presence of GUS-stained mycelium of the pathogen viewed microscopically within infected plant organs provided unequivocal evidence that F. subglutinans is indeed a causal agent of mango malformation disease.
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