ISHS Acta Horticulturae 509: VI International Symposium on Mango
EXO-POLYGALACTURONASE FROM RIPE MANGO (MANGIFERA INDICA LINN CV. NAM DOK MAI) |
| Authors: | P. Chaimanee, N. Lertwikoon, L. Bungaruang, O. Suntornwat |
| Keywords: | polygalacturonase, ripening process, mango |
| Abstract:
Polygalacturonase (PG) is the most studied cell wall hydrolases. Both exo-PG (EC 3.2.1.67) and endo-PG (EC 3.2.1.15) were detected during the ripening process of mango. The increase in exo-PG was highly correlated with ripening whereas endo-PG activity was constant. Exo-PG was extracted from ripe mango mesocarp tissue. The enzyme has the relative molecular weight of 66 k and 58 k as detected by SDS-polyacrylamide gel electrophoresis. In Northern blot analysis, there was barely detectable signal with the PG probe. | |
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