ISHS Acta Horticulturae 508: XIX International Symposium on Improvement of Ornamental Plants
DIVERSITY OF NUCLEAR DNA CONTENTS OF HYDRANGEA |
| Authors: | D. Demilly, C. Lambert, H. Bertrand |
| Keywords: | Hydrangea, ploïdy level, triploïd, flow cytometry |
| Abstract:
Information about Hydrangea ploidy and DNA content is poor. Species are described as diploid 2x = 36 (Sax 1931, Sugiura 1931). This study was carried out to characterise Hydrangea species and cultivars from the French collection settled in Angers. Flow cytometry was used with a monoparametric cytometer, using DAPI (6-diamino-2phenylindole, A-T specific fluorochrome). Fluorescence of Hydrangea nuclei was compared with an internal reference of Pisum nuclei. Direct determination of chromosome number from root tips with a microscope made possible the calibration of the flow cytometer. | |
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