ISHS Acta Horticulturae 508: XIX International Symposium on Improvement of Ornamental Plants
INITIATION AND ESTABLISHMENT OF LONG-LIVED CALLUS AND SUSPENSION CULTURES OF TULIPA PRAESTANS |
| Authors: | I. Famelaer, E. Ennik, J. Creemers-Molenaar, W. Eikelboom, J.M. van Tuyl |
| Keywords: | Cold treatment, 4-methylene-glutamic acid, silver nitrate, embryogenesis, root morphogenesis |
| Abstract:
Results on callus induction and proliferation in Tulipa praestans are presented. Cold treatment, at 4°C, of seeds from a cross between T. praestans plants from different origins, affected the rate and frequency of callus induction and the amount and location of callus formation on embryos. Cold-treated embryos proved to be the most suitable explants for callus induction and proliferation. Different callus types were initiated from immature and mature embryos, which received a short (11 week) and long (17 week) cold-treatment respectively. Only a nodular, compact, yellowish callus (type 2) showed sustained proliferation. Root morphogenesis occurred, but no shoot primordia developed from this callus. Suspension cultures were established from type 2 callus. The influence of cold treatment, light and darkness, culture media, growth regulators and 4-methylene-glutamic acid on the initiation and establishment of long-lived callus and suspension cultures is discussed. | |
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