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ISHS Acta Horticulturae 502: II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology

TRITERPENES AND PHENOLICS IN CALLUS OF MAYTENUS AQUIFOLIUM MART.

Authors:   S.C. França, I.B. Duarte, A.M.S. Pereira, D. Carvalho, M.E.C. Queiroz
Keywords:   tannins, friedelin, friedelin-3-ol, anti-ulcer, espinheira santa, Maytenus aquifolium
Abstract:
The antiulcerogenic effects of Maytenus aquifolium Mart. extracts have been confirmed by biological assays and are apparently related to total phenols, more specifically tannins and triterpenes. Leaves of M. aquifolium have been widely consumed in Brazil as a tea against ulcer. The present work reports the production of M. aquifolium callus on semi-solid media and cell suspension cultures. Both were evaluated for their ability to accumulate triterpenes and phenolics. Dried cells were extracted and phenolics and triterpenes were analyzed by spectrometry and gas chromatography. Friedelan-3-ol was detected in cell suspension and friedelin in callus. Cells cultured in semisolid medium accumulated higher yields of phenolics than cell suspensions.

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