ISHS Acta Horticulturae 502: II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology
MICROPROPAGATION OF ARISTOLOCHIA FIMBRIATA CHAM. |
| Authors: | C. Bravo, G. Yormann, B. Llorente |
| Keywords: | Aristolochia fimbriata, Aristolochiaceae, aristolochic acids, patito |
| Abstract:
A method for micropropagation of Aristolochia fimbriata Cham. is described. The basal medium contained the Murashige-Skoog's salts (MS), Gamborg's vitamins and sucrose. One-node stem segments were initiated on the basal medium supplemented with 0.1 mg l-1 gibberellic acid (GA3), 2.5 mg l-1 6-benzyl-amino purine (BAP) and indole-3- butyric acid (IBA) at different concentrations, achieving 5-fold shoot multiplication at 14 days with 0.25 mg l-1 IBA. The formed shoots were sectioned and transferred to the basal medium with BAP (1.0 mg l-1) where 8-fold shoot multiplication was obtained. For root formation, shoots were cultured on the basal medium with half the MS macronutrients, supplemented with IBA or a-naphthalene-acetic acid (NAA). The IBA supplementation produced roots in all the tested concentrations, while NAA only led to callus formation. | |
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