ISHS Acta Horticulturae 502: II WOCMAP Congress Medicinal and Aromatic Plants, Part 3: Agricultural Production, Post Harvest Techniques, Biotechnology
IN VITRO CLONING OF THYMUS MASTICHINA L. FIELD-GROWN PLANTS |
| Authors: | M.L. Mendes, A. Romano |
| Keywords: | Aromatic plants, micropropagation, thyme |
| Abstract:
In vitro vegetative propagation of thyme (Thymus mastichina L.) has been obtained from mature field-grown plants. Nodal segments were cultured on Murashige and Skoog (1962) modified medium supplemented with 0.1 mg.l-1 BAP. Establishment of cultures was difficult due to the high incidence of contaminations. Cultures were multiplied in Collet (1985) modified medium. The effects of BAP, 2iP, K, and BAP+IAA on shoot growth and multiplication were investigated. BAP was the best cytokinin during multiplication phase. The effect of the auxins IAA, IBA and NAA, on the in vitro rooting of the shoots was studied. The rooting percentage was high for all the auxins tested. The best results for root number, longest root length and shoot length were observed with 1 mg.l-1 NAA. One hundred per cent of plantlets were successfully acclimatized under controlled conditions with high relative humidity. | |
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