ISHS Acta Horticulturae 498: IV International Symposium on Kiwifruit
STRUCTURAL AND MICROANALYTICAL STUDIES ON FROZEN-HYDRATED TISSUES FROM DIFFERENT KIWIFRUIT ORGANS |
| Authors: | C. Vitagliano, L. Sebastiani, A. Minnocci |
| Keywords: | Actinidia deliciosa, calcium, LTSEM, Microanalysis, ion localisation, potassium |
| Abstract:
The analysis of ion distribution and accumulation at various time of plant development and for different physiological conditions in fruit tree organs is fundamental to understand the quali-quantitative mineral requirement in order to enhance fruit production. Biochemical assays cannot easily provide the spatial localisation of mineral elements distribution and storage at cell-tissue level. To overcome these problems and provide a reliable pattern of ion content and distribution, cryofixed samples maintained in their frozen-hydrated state can be analysed by Low Temperature Scanning Electron Microscopy (LTSEM) and EDX X-ray. In this article, some preliminary structural and analytical investigations performed with LTSEM and EDX X-ray microanalysis on frozen-hydrated kiwifruit leaf, peduncle and fruit samples will be presented. | |
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