ISHS Acta Horticulturae 492: I International Symposium on Cucurbits
INDUCTION OF PARTHENOGENETIC EMBRYOS BY IRRADIATED POLLEN IN CUCUMBER |
| Authors: | M. LOTFI, A. KASHI, R. ONSINEJAD |
| Keywords: | Cucumis sativus, haploid, gynogenesis |
| Abstract:
Two greenhouse cutivars (Rubah, RZ) and one field cultivar (Daminus) were used as maternal genotype and one local open pollinated cultivar was used as pollen source. Male flowers collected four times were exposed to gamma radiation at 100, 200, 300 and 400 Gy. The following day pollination was performed with those irradiated pollen. Irradiation had no effect on pollen germination percent. These pollen caused fruit set naturally with empty seeds. Only some of seeds included an embryo without endosperm which seemed to be haploid resulting from parthenogenesis and furthermore some of them included a gelatinous tissue. The embryos were rescued and cultured on E20 medium. Some of these embryos were reached to cotyledonary stage while others were in globular or heart stage. Heart shape embryos had good ability for developing to complete plant. The cultivars had no effect on the rate of induced haploid embryos and obtained plants. Among the irradiation doses 100 Gy caused the maximum embryo induction, howere all of these embryos were not in suitable conditon for developing. In 100 Gy treatment two complete seeds also were formed. | |
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